Figure 3

miRNA overexpression kinetics. A375 cells were infected with either let-7a or miR-372-encoding virus and expression was measured by miRNA qPCR. (A, B) Expression of let-7a and miR-372 is measured 0, 4, 12, 24 and 72 hours after infection. Data is plotted as fold overexpression (A) or relative to U6 snRNA expression (B). (C) Three days after infection, samples were passaged and cultured either with or without puromycin selection. Expression of let-7a and miR-372 was measured 5, 9 and 20 days after infection. For both miRNAs, expression dipped after three days but stabilized thereafter, resulting in a sustained overexpression.