Figure 4

Lentiviral transduction of miRNAs in a high-content setup. Five different cell lines were infected with either empty vector miR-372, or let-7a. Cells were fixed 96 hours after infection and stained with Hoechst. Samples were analyzed with automated image analysis software. Four parameters were assessed: 1, number of nuclei per field; 2) nuclear size; 3) nuclear shape; 4) chromatin condensation. Graphs on the right show the quantification of the number of nuclei per field. Error bars represent standard deviations of triplicate wells. Four fields were counted per well.