Figure 7

The txnip and trib3 promoters contain potential c-Jun binding sites. A, DNA sequences of functionally important ATF sites known to bind c-Jun and ATF2 in the c-jun, dp5 and mkp1 promoters. The c-jun promoter contains two ATF sites, the jun2 and jun1 TREs, the dp5 promoter contains one site, and the mkp1 promoter contains two sites. Each of these sites has been shown to bind c-Jun and ATF2 in ChIP assays and to be important for promoter induction after NGF withdrawal [24, 26, 34]. The orientation of the sequences is 5' to 3'. B, A conserved ATF site was identified in the txnip promoter using CONSITE software (http://asp.ii.uib.no:8090/cgi-bin/CONSITE/consite) to analyse orthologous pairs of genomic sequences. This sequence (5'-TGAGGTAA-3') is identical to the reverse complement of the jun2 TRE (5'-TTACCTCA-3') in the c-jun promoter. Numbers refer to position in relation to Exon 1 of the rat txnip gene, as defined in the NCBI entry for rat txnip (http://www.ncbi.nlm.nih.gov/gene/117514). * indicates nucleotides conserved in all four species. Two non-conserved nucleotides in the mouse ATF site are shown in red. C, A conserved ATF site was identified in the trib3 promoter using CONSITE software to analyse orthologous pairs of genomic sequences. This sequence (5'-TTACATCA-3') is identical to the reverse complement of the ATF site in the dp5 promoter. Numbers refer to position in relation to Exon 1 of the rat trib3 gene, as defined in the NCBI entry for rat trib3 (http://www.ncbi.nlm.nih.gov/gene/246273). * indicates nucleotides conserved in all four species. One non-conserved nucleotide in the human ATF site is shown in red.