Figure 21

Immunoflourescence images taken from sections of ovarian follicles showing the protein of IRF6 expressed only in oocytes and that of MSX1 expressed in both oocyte and CCs. Lanes A and E. = ovarian sections incubated with IRF6 and MSX1 primary antibodies showing the location of IRF6 and MSX1 proteins, respectively. B and F = Protein and nuclear staining showing IRF6 and MSX1 proteins and DNAs together. C = Sections incubated only with DAPI showing the nucleus of the cells D. Negative control staining in which the sections were incubated only with secondary antibodies. The red arrows indicate the location of each protein. Scale bars below each picture represent 20 μm