Table 1 Pairs of primers that were used for validation of array data
Gene name | Accession number | Forward (5' - 3') | Reverse (3' - 5') | Annealing temperature (°C) | Product length (bp) |
|---|---|---|---|---|---|
GDF9 | NM_174681 | AGCGCCCTCACTGCTTCTATAT | ACACCCTCAGCAGCTTCTTCTC | 57 | 152 |
MSX1 | NM_174798 | AAGGTATCCACAGTCCCCAGC | TCTGCCTCTCCTGCAAAGTTC | 56 | 180 |
IRF6 | NM_001076934 | GGACTCCAAACGCTTCCAGA | TCCTTGGTGCCATCATACATCA | 54 | 212 |
SPARC | NM_174464 | CGATGATGGTGCTGAGGAAA | TGGTGGCAAAGAAGTGGCA | 53 | 220 |
HPSE | NM_1744082 | ATGGGCATAGAAGTGGTGATGA | TGTTTGGTGTTTGTGCAATGAA | TD 54-50 | 202 |
DGAT2 | NM_205793 | TGAACCGGGACACCATAGACTA | CACCTCATTCTCCCCAAAGGA | 55 | 205 |
SOX2 | NM_001105463 | GCGGCAACCAGAAGAACAG | GCTTCTCCGTCTCGGACAAA | 55 | 170 |
HAS2 | NM_174079 | CCAAATGAAATGCCAAAGGAA | CAACGTCAACCAAGCTTCACA | TD 54-50 | 237 |
PTX3 | NM_001076259 | TTTATTCCCCATGCGTTCCA | CTCCACCCACCACAAGCATT | 53 | 205 |
IGF2BP3 | XM_588560 | GACGCGAAAGTGAGGATGGT | TGCACTTGACAAATTCTGGAGC | 54 | 215 |
ADAMTS1 | NM_001101080 | TCGTCATACAGCTCCCCTCC | ATTGACACACCATTTCCCCTCT | TD 57-54 | 220 |
PDK4 | XM_583960 | ATTTTTGCGACAAGAGTTGCCT | GGATTCCTTGTGCCATTGTAGG | TD 56-52 | 250 |
CCRK | XM_879150 | GGCCCCACTCATGGCTACTT | TCCTGAGGGTGATGCTGGTAA | 57 | 170 |
FOSB | XM_880646 | TTCCTGAATCTCTCCCGCC | TGCTCACAGCCTCACACTCG | 56 | 205 |
POU5F1 | NM_174580 | AGAAGGGCAAACGATCAAGC | GGTGACAGACACCGAGGGAA | TD 57-53 | 205 |
VEGFA | NM_174216 | GGTTTCGGGAACCAGACGT | GGCAATCCAATTCCAAGAGGA | 55 | 233 |
18S | NR_003286 | GTGCCCTTCCGTCAATTCCT | ACGAAAGTCGGAGGTTCGAA | 55 | 184 |
GPT | XM_585516 | CCGATGAGGTGTACCAAGACAA | CCATATTCACCACCTCCACGT | 55 | 185 |
DAPL1 | NM_001025346 | AAATTTCCAGCAGTAGCGCAC | AGCTCTCAGACATTCGAGGCA | 55 | 158 |
IRF7 | NM_001105040 | CTCCCCGCACTACACCATCTA | GCCTGTTCCACCTCCATCA | TD 58-55 | 245 |
GADD45A | NM_001034247 | GACCGAAAGGATGGATAAGGTG | TGGATCAGGGTGAAGTGGATCT | 56 | 200 |
DPP4 | NM_174039 | CAACTGGGCTACTTACCTTGCA | TTACGTACCCTCCGTATGACCA | 56 | 223 |
IFI6 | NM_001075588 | ACGGTGACAAAGCCTTGAGC | AGGTCACCATGCCCCAGAA | 55 | 158 |
ADAMTS4 | NM_181667 | CCATTGTGGAGGATGATGGG | AGGAAGTCAGTGATGAAGCGG | 55 | 210 |
CASP1 | XM_592026 | CTCACTCAGAGCATCGGACCT | GTTTACCCATACCATCCCTTGC | 57 | 229 |
IFIT5 | NM_001075698 | CACAGTGTATCGGCTGGATGA | GGTTGGGCTGATATCTGGTCC | 56 | 200 |
DDX39 | NM_001034752 | GCAGTTCAAGGACTTCCAGC | GCTCTGCCACATTCACTTCA | TD 55-52 | 228 |
GTF2A2 | NM_001037619 | AGAAAGAGGTTCTGCCCGGA | CTTATCGGCTGCATTGAAAGC | 55 | 150 |
TSSC1 | XM_001789233 | AGCAGCGACAGCAGAGTCATC | TAGCTCAGGGAGGCGAACAG | 57 | 229 |
FSHR | NM_174061 | GCTGGATCTTTGCTTTTGCAGT | ATGCGCTTGGCTATCTTGGT | 54 | 243 |
GPT | NM_001083740 | CCGATGAGGTGTACCAAGACAA | CCATATTCACCACCTCCACGT | 56 | 185 |
PLAUR | NM_174423 | GGATTCCACAACAACCACACCT | TCGCTTCCAGACATTGATTCAT | TD 56-52 | 204 |
CA2 | NM_178572 | CAAAGCAGTGCTGAAAGATGGA | AAAACACCCACAACAGCCAGTC | 55 | 212 |