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Table 1 Comparison of fold changes between microarray and qPCR

From: Genome-wide differential gene expression in immortalized DF-1 chicken embryo fibroblast cell line

Accession No. Gene Symbol Microarray qPCR
AB109635 HMGCR -1.03 -0.81
AB196971 APCDD1 4.75 7.96
AF505881 SCX -3.27 -1.94
AJ131110 TWIST2 4.61 6.77
BU279212 CBLN2 7.84 8.64
BX929635 NDP -4.26 -1.64
BX931599 VIPR2 -2.90 -11.34
BX932694 MPZL2 5.51 4.16
BX933478 MXRA5 -2.69 -7.97
BX933888 C1QTNF3 -2.53 -2.28
BX935456 EGLN3 -3.04 -4.48
BX936211 TMEM116 4.26 5.94
CK611983 CSTA 4.49 7.92
CR385566 CLEC3B -4.46 -3.58
D87992 ANPEP -4.25 -6.29
M60853 THBS2 -5.15 -5.28
M64990 PTGS2 -6.78 -10.36
M80584 LUM -3.14 -4.59
M87294 NPY -3.73 -7.15
X87609 FST -4.12 -10.04
  1. The gene expression levels of 20 genes from microarray analysis were confirmed by qPCR. The expression levels were presented by fold changes values in microarray analysis, while, for qPCR, the values were calculated by 2-ΔΔCT method, which were comparable to fold changes in the microarray. All values are mean values determined by the calculations from three replicate assays.