Phylogenetic analysis of the sequences amplified for the gag fragment. All amplified sequences from genomic DNA of Oregon-R and GIII (names in green and orange, respectively) and from total RNA from 3rd instar larvae of Oregon-R and GIII (names in blue and red, respectively) were used. Also included are the previously annotated HeT-A sequences containing the analyzed fragment (shown in black). A. Phylogenetic tree constructed using all obtained sequences. The different subfamilies are indicated with boxes with the corresponding letter. Additionally, the tree includes a Drosophila yakuba HeT-A sequence (AF043258, named yakuba in the tree), in order to illustrate the degree of variability of HeT-A inside species and between species (D.yakuba and D.melanogaster are considered to be ~10 million years of genetic distance; source: http://flybase.org). The position of sequence G3-gag-69 is indicated. B. Bar chart representing the relative abundance of each subfamily.