Table 1 Comparison between RNA-seq [17] (48 h) and microarray data for Chlamydomonas reinhardtii cultures incubated under nitrogen starvation conditions For microarray data average values for important genes during 96 h starvation are displayed (24 h, 48 h, 72 h, 96 h).
From: Construction and evaluation of a whole genome microarray of Chlamydomonas reinhardtii
Reporter Identifier | Reporter Name | Reporter Description | Log2 ratio microarray | Log2 ratio RNA seq |
|---|---|---|---|---|
nitrogen related genes | ||||
182971 | NSG13 | "nitrogen-starved gametogenesis 13"... | 5.0 | 3.6 |
184661 | NIT1 | "nitrate reductase"... | 4.6 | 7.9 |
192085 | NII1 | "Nitrite reductase"... | 4.4 | 7.7 |
205647 | NIT2 | "transcription factor regulating nitrogen metabolism"... | 1.5 | 2.6 |
188890 | "NADH nitrate reductase"... | 3.0 | 5.2 | |
156131 | AMT4 | "Ammonium transporter"... | 5.1 | 7.2 |
158745 | AMT1 | "Ammonium transporter"... | 3.1 | 5.5 |
133971 | GLN1 | Glutamine synthetase | 0.9 | 1 |
129468 | GLN2 | Glutamine synthetase | 0.1 | -0.3 |
136895 | GLN3 | Glutamine synthetase | 6.5 | 6.6 |
147483 | GLN4 | Glutamine synthetase | 2.3 | 1 |
photosynthetic related genes | ||||
184724 | LHCSR1 | "stress-related chlorophyll a/b binding protein 1"... | -2.4 | -1.2 |
184731 | LHCSR2 | "Stress-related chlorophyll a/b binding protein 2"... | 3.7 | 2.6 |
184730 | LHCSR3 | "Stress-related chlorophyll a/b binding protein 3"... | 3.8 | 1.7 |
196341 | PSBS1 | "chloroplast Photosystem II-associated 22 kDa protein"... | 2.0 | 6.9 |
171516 | PSBS2 | "chloroplast Photosystem II-associated 22 kDa protein"... | -0.3 | |
196341 | PSBX | "4.1 kDa photosystem II subunit"... | -2.1 | 6.9 |
205940 | PSAL | "Photosystem I reaction center subunit XI"... | -2.4 | 0.0 |
192478 | PSAK | "photosystem I reaction center subunit psaK"... | -2.3 | -3.4 |
184810 | LHCB4 | "chlorophyll a-b binding protein of photosystem II"... | -2.4 | -2.3 |
187025 | LHCA8 | "light-harvesting protein of photosystem I"... | -2.2 | -4.6 |
192961 | LHCA7 | "light-harvesting protein of photosystem I"... | -2.5 | -5.0 |
186299 | LHCA6 | "light-harvesting protein of photosystem I"... | -2.7 | -5.4 |
153678 | LHCA4 | "light-harvesting protein of photosystem I"... | -2.6 | -4.5 |
206001 | LHCA1 | "light-harvesting protein of photosystem I"... | -3.2 | |
184397 | LHCBM3 | "Light-harvesting complex II chlorophyll a-b binding protein M3"... | -2.0 | -3.7 |
184479 | LHCBM9 | "chlorophyll a-b binding protein of LHCII"... | -0.2 | -1.5 |
126810 | NAB1 | "nucleic acid binding protein"... | -1.6 | -4.1 |
other genes | ||||
185841 | RDP3 | "Rhodanese domain phosphatase"... | 2.4 | -3.4 |
196438 | PTB5 | "sodium/phosphate symporter"... | 5.3 | 0.6 |
196465 | PTB5 | "sodium/phosphate symporter"... | 4.3 | 1.7 |
183357 | PTB3 | "sodium/phosphate symporter"... | 3.8 | -0.6 |
96789 | GPD4 | "Glycerol-3-Phosphate Dehydrogenase/Dihydroxyacetone-3-Phosphate Reductase"... | 2.8 | 1.4 |
146945 | GPD1 | "Glycerol-3-Phosphate Dehydrogenase/Dihydroxyacetone-3-phosphate Reductase"... | 3.6 | 4.1 |
182461 | GLD2 | "glucose-6-phosphate dehydrogenase"... | 2.3 | 4.1 |
191987 | ARG1 | "N-acetyl-gamma-glutamyl-phosphate reductase"... | 2.8 | 0.8 |
R:191668/1 | ICL1 | "isocitrate lyase" | 0.1 | -5.8 |