Insertion in the non-edited ND4 gene in Sylvio X10. A) Partial representation of the ND4 nucleotide alignment from T. cruzi strains Sylvio X10, CL Brener and Esmeraldo. SNPs are indicated by 's' above the alignment; the distribution of SNPs is compiled in Table 3. The Sylvio X10 strain has an insertion ('i' under the alignment) at position 656. B) The ND4 amino acid alignment from the three T. cruzi strains; the Esmeraldo sequence is partial due to a 99-nt deletion in the 5' end. An early stop codon (#) is present at position 226 in the Sylvio X10 strain. The available Esmeraldo translation is included for comparison. C) Deletion of the extra A in the Sylvio X10 ND4 gene restores the truncated portion of the protein from aa 226.