Phylogenetic distribution of species of top unique BLAST hits for the P. hawaiensis transcriptome sequences. First, raw reads that contained C2H2 zinc finger sequences were removed before assembly. Second, assembled sequences that contained C2H2 zinc finger sequences were removed from the output file. We then determined the unique BLAST hits for the remaining non-redundant assembly products (isotigs + singletons). 90% of the resulting unique BLAST hits were from species belonging to the clades shown. Blue circles are scaled according to the proportion of sequences with species belonging the clade indicated as their top BLAST hit. Over 50% of top BLAST hits are from arthropod species. Phylogenetic tree modified from [63, 74, 75]. Hits from the following most abundant species are represented: D. mojavensis, D. willistoni, D. ananassae, D. grimshawi, D. pseudoobscura pseudoobscura, Aedes aegypti, Anopheles gambiae, Culex quinquefasciatus (Flies & Mosquitoes), Bombyx mori (Moth), Tribolium castaneum (Beetle), Harpegnathos saltator, Camponotus floridanus, Apis mellifera, Nasonia vitripennnis (Bee, Wasp & Ants), Acyrthosiphon pisum (Aphid), Pediculus humanus corporis (Louse), Ixodes scapularis (Tick), Penaeus monodon, Lepeophtheirus salmonis, Litopenaeus vannamei (Crustaceans), Caenorhabditis remanei (Nematode), Gallus gallus, Taeniopygia guttata (Birds), Rattus norvegicus, Mus musculus, Monodelphis domestica (Mammals), Xenopus laevis, X. tropicalis (Amphibian), Danio rerio, Tetraodon nigroviridis (Fish), Branchiostoma floridae (Amphioxus), Ciona intestinalis (Sea Squirt), Saccoglossus kowalevskii (Acorn Worm), Strongylocentrotus purpuratus (Sea Urchin), Trichoplax adherens (Trichoplax), Hydra magnipapillata, Nematostella vectensis (Cnidarians), Perkinsus marinus (Dinoflagellate). See Additional File 5 for details.