Methylation analysis of VLR-regulated genes. A and B, COBRA and bisulfite-modified DNA sequence analysis, respectively. A, values on the y-axis are 2-ΔCP in comparison with the average of mock digestion triplicates. An increase in methylation results in relatively low 2-ΔCP values. Black bars, control; white bars, VLR-stimulated. Bars, standard deviations. B, numbers on the vertical axis indicate the position of the analyzed sequence relative to the transcription start site (bp). Ticks represent CpG dinucleotides. Horizontal bars represent the extent of methylation of the corresponding residues in the clones analyzed.