The association of DNA damage response and nucleotide level modulation with the antibacterial mechanism of the anti-folate drug Trimethoprim

Table 1 Treatment regimens used for gene expression analysis

Condition	Base medium	Supplementation	Viability loss
LBTMP	Luria-Bertani broth	-	+ (90% loss in 3 hours)
M9TMP	Minimal M9	None	-
M9TMPAdAA	Minimal M9	Met, Gly, Ad	+ (>70% loss in 6 hours)
M9TMPAA	Minimal M9	Met, Gly	-
M9TMPAd	Minimal M9	Ad	-
M9TMPThyAdAA	Minimal M9	Met, Gly, Ad, Thy	-

A list of treatment regimens used. Viability loss indicates the relative magnitude of cell lethality (+: maximum, -: no loss). TMP: Trimethoprim (50 or 25 μg/ml), Met: methionine, Gly: glycine, Ad: adenine (each at 50 μg/ml final concentration)

ISSN: 1471-2164