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Table 2 List of mutants yielding high biomass in presence of TMP at sub-lethal concentrations in LB medium

From: The association of DNA damage response and nucleotide level modulation with the antibacterial mechanism of the anti-folate drug Trimethoprim

Resistant knock-out candidates

Gene

Gene ID

Description

moaE

b0785

Molybdopterin (MPT) synthase, large subunit; chlorate resistance; dimer of dimers with MoaD

ybhN

b0788

Function unknown

yliE

b0833

Function unknown

ymfQ

b1153

Function unknown

yobG

b1826

Function unknown

yecN

b1869

Function unknown

crr

b2417

Phosphocarrier protein for glucose of the PTS; Enzyme IIA(Glc); formerly EIII(glc)

recJ

b2892

Single-stranded DNA-specific exonuclease, 5'-3'

ygjT

b3088

Function unknown; induced by alkali; putative membrane transport or efflux protein

ygjU

b3089

Na+/serine (threonine) symporter

nanA

b3225

N-Acetylneuraminate lyase (aldolase)

yhiP

b3496

Function unknown

uhpT

b3666

Fosfomycin sensitivity; sugar P transport system; transport protein for hexose P's

yiiQ

b3920

Function unknown

uvrA

b4058

Excision nuclease subunit A; repair of UV damage to DNA; LexA regulon; binds Zn(II)

fimE

b4313

Site-specific recombinase for fimA promoter segment inversion; bias for ON to OFF phase switching

deoC

b4381

Deoxyribose-phosphate aldolase; deoxyriboaldolase; binds selenium

deoA

b4382

Thymidine phosphorylase

  1. A total of 18 mutants, based on their relatively high biomass yield in presence of TMP, were identified as sensitive candidates. Genes whose functions are related to DNA maintenance and nucleotide salvage pathway are highlighted in bold. Genes are sorted by their Gene IDs.