The association of DNA damage response and nucleotide level modulation with the antibacterial mechanism of the anti-folate drug Trimethoprim

Table 3 List of mutants yielding low biomass in presence of TMP at sub-lethal concentrations in LB medium

Sensitive knock-out candidates
Gene	Gene ID	Description
deoR	b0840	Repressor for deo operon, nupG and tsx; binds deoxyribose-5-phosphate inducer
flgH	b1079	Flagellar synthesis, basal body L-ring protein
fliD	b1924	Hook-associated protein 2, axial family; flagellar regulon
fliI	b1941	Cytoplasmic membrane ATPase involved in flagellar assembly; involved in export of flagellar axial protein subunits
fliN	b1946	Flagellar switch protein
JW5799	-
mdoH	b1049	Membrane glycosyltransferase
tdk	b1238	Deoxythymidine kinase
ycbC	b0920	Function unknown
yciM	b1280	Function unknown
ydhA	b1639	Function unknown
yfgE (hda)	b2496	Required for regulatory inactivation of DnaA; multicopy suppressor of dnaN(ts)
alaS	b2697	Alanine--tRNA ligase

A total of 13 mutants, based on their relatively low yields, were identified as sensitive candidates. Genes whose functions are related to DNA maintenance and nucleotide salvage pathway are highlighted in bold. Genes are sorted by their Gene IDs.

ISSN: 1471-2164