Figure 2

Pathotyping scheme of 55 C. jejuni strains ordered according to STs. Each of ten groups represents one ST and contains at least one strain from human, one strain from animal and one strain from food sources. The distribution of putative virulence- and host-associated genes was tested by PCR (primer combinations in Table 2). The presence of genes Cj1305 and Cj1306 was detected using a combination of primers in Cj1303 and Cj1307, by PCR product length: 1 indicates the presence of just one gene, either Cj1305 or Cj1306, and 2 indicates the presence of both genes. Color-coding: green: gene present; red: gene not present; yellow: weak PCR product; white: not tested. Gene copies marked by asterisks were additionally sequenced to identify interstrain polymorphisms using Sanger sequencing.