Figure 4

Differential NF-κB activation or inhibition by various ST-21 C. jejuni strains in mammalian cells. Stably transfected Caco-2 cells (A) and HD-11 cells (B) containing a NF-κB-luciferase gene fusion were coincubated with 8 different C. jejuni strains (from human (h), chicken (ch), bovine (b) and food (f) source) at an MOI of 50 in triplicates and coincubated for 1 h. The values are depicted in percent relative to the mock-infected sample, which was set to 100%. One representative experiment of four is displayed. Statistics was performed using a Student's t test. A p-value of < 0.05 was considered statistically significant (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Selected statistical evaluation of differences between strains from one source or between two different sources is shown.