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Figure 5 | BMC Genomics

Figure 5

From: Closely related Campylobacter jejuni strains from different sources reveal a generalist rather than a specialist lifestyle

Figure 5

Differences between ST-21 C. jejuni strains in metabolic activity and energy levels. (A) Carbon source utilization by eight ST-21 strains (left panel) from different sources (h = human; ch = chicken; b = bovine; f = food) and nine human-derived strains (from left to right: xy898, R267, xy904, A396, 222, 02551, 02557, A412 and A599) of different STs (right panel) were tested at 42°C using BioLog Phenotype Microarray PM1 plates (area values). Grey color indicates no or only weak substrate utilization for all strains (all area values below 800) and green indicates high substrate utilization for all strains (all area values above 800). The colors pink, yellow, beige and blue indicate strain-specific utilization: pink-zero utilization, beige-< 1,000, yellow-< 9,000, blue-> 9,000. The six bordered rectangles on the left panel (from top to bottom, second from left column) indicate the substrates L-galactonic acid-g-lactone, tyramine, m-hydroxy-phenylacetic acid, p-hydroxy-phenylacetic acid, glycyl-L-aspartic acid, glycyl-L-glutamic acid and glycyl-L-proline, which are not used by some strains (phenotypic variant 1). PM1 experiments with ST-21 strains were performed at least three times independently on different days. Shown for each substrate are the mean area values of three independent experiments performed at 42°C for 30 h incubation time. PM1 experiments for other STs were performed either once or twice (mean values shown for ST-45 and ST-267 strains) at 42°C. (B) Differences in intracellular ATP levels (energy harvest) of eight ST-21 strains from different sources. Statistics was performed using a Student's t test. A p-value of < 0.05 was considered statistically significant (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).

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