Figure 1

Density distribution of reads from bidirectional and strand-specific libraries of late trophozoite, schizont, gametocyte II, and gametocyte V. The sense fraction was calculated based on the ratio between sense and total number of reads present at each exon in the genome (total of 14,777 exons). Density represents the number of genes that have a particular ratio of forward reads over total number of reads for that gene. Strand-specific libraries (solid lines) show directionality characterized by high frequencies of exons with fraction values close to 1, whereas those from bidirectional libraries (dashed lines) have the majority of exons with sense fractions between 0.2 and 0.5.