Directional gene expression and antisense transcripts in sexual and asexual stages of Plasmodium falciparum

BMC Genomics

Table 1 Summary of sequence reads obtained from different RNA-Seq libraries

Library	Total reads	Mapped reads	Unmapped reads	% mapped reads
R_BD¹	13,456,136	4,557,242	8,898,894	33,86%
ET_ BD ¹	14,505,855	5,801,368	8,704,487	39,99%
LT_ BD ¹	14,599,502	7,567,175	7,032,327	51,83%
Sc_ BD ¹	9,861,818	1,487,604	8,374,214	15,08%
GII_ BD ¹	9,294,474	3,647,906	5,646,568	39.25%
GV_ BD ¹	10,641,921	6,610,820	4,031,101	62.12%
Oo_ BD ²	55,140,294	714,769	54,425,525	1.3%
LT_SS³⁺	13,928,058	8,852,802	5,075,256	63.56%
Sc_ SS ³⁺	14,360,448	8,386,042	5,974,406	58.40%
GII_ SS³⁺	9,331,268	4,952,677	4,378,591	53.08%
GV_ SS³⁺	20,197,230	6,534,612	13,662,618	32.35%

R, ET, LT, Sc, GII, GV, and Oo correspond to ring, early trophozoite, late trophozoite, schizont, gametocyte II, gametocyte V, and ookinete stages, respectively. BD represents bidirectional libraries, and SS represents strand-specific libraries. "Total reads" corresponds to the initial output of sequencing after analysis with Illumina pipeline v3.1; "mapped reads" refers to numbers of reads mapped to the Pf3D7 reference genome (PlasmoDB version 7.1) allowing one single hit and no mismatches ⁽¹⁾, one mismatch ⁽²⁾, or up to two mismatches ⁽³⁾ at the reads. ⁺ indicates the total numbers of reads obtained after de-multiplexing.

ISSN: 1471-2164