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Table 1 Next generation sequences used for SNP discovery and for estimation of sequencing error rates

From: Annotation-based genome-wide SNP discovery in the large and complex Aegilops tauschii genome using next-generation sequencing without a reference genome sequence

Accession

Sequencing platform

Sequence type

Total reads

Total size (Mb)

Average Read length (bp)

Genome coverage

AL8/78

Roche 454 GS-FLX Titanium

Shotgun genomic

14,087,315

5,445

380.5

~1.35X(a)

AS75

Roche 454 GS-FLX Titanium

Shotgun genomic

1,394,433

433

310.8

~0.11X

AS75

Illumina Solexa

Shotgun genomic

74,814,052

6,284

84

~1.56X

AS75

AB SOLiD v3.0

Shotgun genomic

2,136,678,966

106,834

50

~26.57X

AS75

AB SOLiD v3.0

Shotgun cDNA

442,086,124

22,104

50

~22X(d)

AL8/78

Roche 454 GS-FLX Titanium

13 BACs(b), genomic

58,971

24.7

418.9

~12.93X

AL8/78

Illumina Solexa

13 BACs, genomic

5,586,903

223

40

~116.75X

AL8/78

AB SOLiD v2.0

13 BACs, genomic

26,013,814

624

25(c)

~326.87X

  1. (a) Genome sizes were estimated based on the 4.02 Gb genome size of Aegilops tauchii [22]. (b) The total size of the 13 BACs is 1.91 Mb. (c) Only 24 bases were used for mapping. (d) Genome coverage was estimated on the basis of average coverage depth of SOLiD cDNA reads mapping to AL8/78 shotgun reads.