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Table 1 Next generation sequences used for SNP discovery and for estimation of sequencing error rates

From: Annotation-based genome-wide SNP discovery in the large and complex Aegilops tauschii genome using next-generation sequencing without a reference genome sequence

Accession Sequencing platform Sequence type Total reads Total size (Mb) Average Read length (bp) Genome coverage
AL8/78 Roche 454 GS-FLX Titanium Shotgun genomic 14,087,315 5,445 380.5 ~1.35X(a)
AS75 Roche 454 GS-FLX Titanium Shotgun genomic 1,394,433 433 310.8 ~0.11X
AS75 Illumina Solexa Shotgun genomic 74,814,052 6,284 84 ~1.56X
AS75 AB SOLiD v3.0 Shotgun genomic 2,136,678,966 106,834 50 ~26.57X
AS75 AB SOLiD v3.0 Shotgun cDNA 442,086,124 22,104 50 ~22X(d)
AL8/78 Roche 454 GS-FLX Titanium 13 BACs(b), genomic 58,971 24.7 418.9 ~12.93X
AL8/78 Illumina Solexa 13 BACs, genomic 5,586,903 223 40 ~116.75X
AL8/78 AB SOLiD v2.0 13 BACs, genomic 26,013,814 624 25(c) ~326.87X
  1. (a) Genome sizes were estimated based on the 4.02 Gb genome size of Aegilops tauchii [22]. (b) The total size of the 13 BACs is 1.91 Mb. (c) Only 24 bases were used for mapping. (d) Genome coverage was estimated on the basis of average coverage depth of SOLiD cDNA reads mapping to AL8/78 shotgun reads.