Figure 10
From: A hybrid BAC physical map of potato: a framework for sequencing a heterozygous genome
Principle of the DNA superpool design of the RHPOTKEY BAC library. Pooled DNAs from 764 quarter library plates (quarter plate pools, QPPs) are each added to a unique combination of four different superpools (SP1...SP90), as shown here for three QPPs. Genetic marker screening is performed on the 90 superpools, and the marker-positive QPPs are then identified by deconvolution of the pooling design (see text for explanation).