In situ hybridization in Drosophila melanogaster larval testes for four differentially expressed genes. CG3767-RA (A-C), CG16910-RA (D-F), CG17934-RA (G-I) and CG4988-RA (J-L) from Wolbachia-infected (C, F, I, L) and uninfected larvae (B, E, H, K). Panels (A, D, G, J) are negative controls using sense probes. The arrows indicate the specific region of signals. All testes were dissected from the 3rd instar larvae and were magnified 200 × under the microscope.