Figure 3

Manual curation and expansion of Schistosoma mansoni GRAFS GPCRs. The transmembrane distributions for the filtered S. mansoni HMM pool is shown before (top) and after (middle) manual editing of the underlying gene models, as predicted by HMMTOP. The number of GPCRs with a predicted intact 7TM domain increases from 27 to 41, coupled with a significant contraction of the distribution spread. The mean TM count shifts from 6.00 to 6.41, which equates to the identification and addition of roughly 42 missing TM domains during the first round of curation. Homology-bases searches against the genome assembly increased the putative 7TM receptor count to 59 (bottom). Receptors in the 8 and 9 TM bin can be considered full-length for our purposes, as the erroneously-predicted additional TM domains can be excised for phylogenetic analysis. Inclusion of these receptors brings the total putative full-length (7TM) receptor tally to 68 (of 117 total sequences).