Table 3 Real time PCR primers used for expression validations of selected enzymes.
Accession | Enzyme | 5'-Forward Primer Sequence-3' | 5'-Reverse Primer Sequence-3' |
|---|---|---|---|
EFI_002990 | AACT-1 | ACTATGCTTGCAGCCCAAAG | ATTTCCCATGCCAACATCAT |
EFI_012483 | AACT-2 | ACAATGCTTGCTGCACAGAC | TCTCCACAAACTCCCATTCC |
EFI_015339 | CS-1 | GGAGAGCTATTTTTGGGCAGT | CGACTTGAGCAAATGAGTCGT |
EFI_018002 | CS-2 | GCAATTGATCCATCAGCAAG | AAGCAAAACAACTCTGGCAAT |
EFI_007143 | DXS-1 | CGCACTAAATTTTGGGTTGC | CAAATCCCTTGGAATTGGTG |
EFI_010574 | DXS-2 | GCTGCAAAAAGCATCACAAA | GGAGCTGGCATTGCTTTTAC |
EFI_003135 | DXS-3 | TTTGCAACAAGTGGCATCTC | ATAGCCAAAGCCTCCACAAA |
EFI_010535 | GGPPS-1 | CAAAAAGCTTCGCAATTCCT | GATTTTTGCGGGTTCTCTGA |
EFI_010585 | GGPPS-2 | ACTTGCAGCCGTTTGTTTCT | ATCAGCAACGAGGGAAAATG |
EFI_008533 | GGPPS-3 | ATTGTTAGCGGGTGCTGAAG | CAGCTCTTCCGCCATTTCTA |
EFI_016937 | GGPPS | TCAATTCGCTGTTCTGCTTC | CCCTTAGAAAGGGCGGAGTA |
EFI_001905 | IDS/HDR | CCACAGACGACTCTGCTTCA | GGTGTGCTCATTCCCATTTT |
EFI_000087 | HDS | GTTTGGGCGATACAATCAGG | ATCCACCTCTTCACCCTCCT |
EFI_0 00846 | HMGR-1 | CTCCACCGCAAAACCTCTTA | AACGACATGGAGAGGAGTGG |
EFI_011656 | HMGR-2 | CAGTGCTGTGAAATGCCTGT | AGCTCTTGTCATGCCATCCT |
EFI_001179 | MDD | GAGACATGGGTGAGGATGGT | CCTCCCCATTAAGCCACATA |
EFI_014705 | Actin-1 | GGGAACGAGTCCCTGGTAGT | CTGCGTTGGTGGTCTTACCT |
EFI_006680 | Actin-2 | AAATATGGCCGACAGTGAGG | ATACCTCGCTTGGACTGAGC |
EFI_000139 | α-tubulin | GGCAACTTTTCCATCCTGAG | TCCAAGACCAGAACCAGTCC |
EFI_014153 | β-tubulin-1 | AAGCAGGTCAATGTGGGAAC | CATTGTCCCTGGTTCAAGGT |
EFI_014578 | β-tubulin-2 | GCTTGCAAGGTTTCCAGGTA | TTTTCCACAAGCTGATGCAC |