Table 4 Putative secreted proteins deduced from the sialotranscriptome analysis and indication of expression by proteomic analysis

Laminin-like

Sg-431|F14→ 2

Amylase/maltase

Sg-214|F16→116, Sg-296|F17→32

Apyrase

Sg-354|F16→58

Sg-126|F16→12, Sg-129|F16→12, Sg-121|F16→12,

Simulium mucin

Sg-120|F16→11, Sg-127|F16→11, Sg-117|F16→10,

Sg-128|F16→10, Sg-119|F16→9, Sg-125|F9→7

Hyaluronidase

Sg-414|F20→14

Diptera secreted protein from conserved insect

Sg-215|F21→134, Sg-216|F21→133, Sg-292|F17→49,

Sg-347|F14→36, Sg-256|F13→28

Simulium collagen-like

Sg-152|F23→87, Sg-149|F23→84

Acid 28-kDa

Sg-320|F23→45, Sg-321|F23→41, Sg-319|F23→23

Long D7

Sg-261|F26→42, Sg-220|F23→24, Sg-218|F23→14

Serine proteases

Sg-244|F24→48, Sg-138|F25→28, Sg-416| F24→6

Antigen-5

Sg-457|F24→30

Aegyptin

Sg-276|F24→37

Simulium basic 28-kDa

Sg-136|F24→34

Deorphanized 19-kDa

Sg-303|F27→17, Sg-309|F27→9

Sv 7.8-kDa

Sg-356|F27→11, Sg-372|F30→9, Sg-227|F32→4, Sg-

205|F31→3

D7 16-kDa

Sg-331|F29→14, Sg-350|F30→9

Simulium basic 13 kDa

Sg-446|F29→2

Deorphanized Sn 8-10 Cys W

Sg-340|F29→22, Sg-324|F29→12

Lysozyme

Sg-263|F30→7

Kunitz domain

Sg-395|F30→2

Sg-1|F30→164, Sg-8|F30→149, Sg-92|F30→53, Sg-

102|F30→43, Sg-94|F30→34, Sg-93|F30→33, Sg-

SVEP

100|F30→32, Sg-95|F30→26, Sg-90|F30→25, Sg-

99|F30→25, Sg-103|F30→22, Sg-101|F30→20, Sg-

344|F30→14

5-Cys Simulium

Sg-282|F30→3

Basic 7-13 Simulium

Sg-403|F30→8, Sg-420|F31→3

Cecropin

Sg-368|F31→2, Sg-369|F31→3

Ultra-short D7

Sg-383|F31→3

Simulium basic 7.4 kDa

Sg-422|F31→4

Deorphanized 8 kDa basic

Sg-258|F31→5

Similar to Kunitoxin

Sg-375|F31→4