A de novo transcriptome of the Asian tiger mosquito, Aedes albopictus, to identify candidate transcripts for diapause preparation

Table 3 Databases used for BLAST homology searches with relevant details for each search

Reference organism	Sequence type	BLAST algorithm	Minimum e-value	Version	Source	Minimum % identity used in final EST set selection
Ae. albopictus	mRNA	BLASTN	1.00E-10	N/A	http://www.ncbi.nlm.nih.gov	85
Ae. aegypti	transcripts	BLASTN	1.00E-10	AaegL1.2	http://www.vectorbase.org	85
Ae. aegypti	genomic	BLASTN	1.00E-10	AaegL1	http://www.vectorbase.org	85
Ae. aegypti	peptides	BLASTX	1.00E-10	AaegL1.2	http://www.vectorbase.org	70
Cx. quinquefasciatus	peptides	BLASTX	1.00E-05	CpipJ1.2	http://www.vectorbase.org	0
An. gambiae	peptides	BLASTX	1.00E-05	AgamP3.6	http://www.vectorbase.org	0
D. melanogaster	translation	BLASTX	1.00E-05	r5.29	http://www.flybase.org	0
Ca. elegans	peptides	BLASTX	1.00E-04	WS218	http://www.wormbase.org	0
Swiss-Prot	proteins	BLASTX	1.00E-03	2010_6	http://www.uniprot.org	0

The minimum e-value used for the initial EST set, and the minimum % identity used for the final EST set, varied based on phylogenetic distance of the organism/database and Ae. albopictus.

ISSN: 1471-2164