A de novo transcriptome of the Asian tiger mosquito, Aedes albopictus, to identify candidate transcripts for diapause preparation

Table 7 Genes verified as DE from qPCR analyses

*Ae. albopictus* ID	Putative homolog	454 category	Fold-change	corrected p-value	Functional category
Aalb_oocyte_rep_c42113_trimmed	phosphoenolpyruvate carboxykinase (pepck)	DI	0.82	0.004	metabolism
Aalb_oocyte_rep_c2808_trimmed	Ecdysone inducible protein L2, putative (eip)	NDI	-0.85	0.005	endocrine signaling
Aalb_oocyte_rep_c41764_trimmed	Inhibitor of growth protein (ing1)	a priori	1.05	0.002	DNA replication/ transcription
Aalb_oocyte_rep_c38864_trimmed	Conserved hypothetical protein (AAEL004873)	NDI	0.48	0.004	unknown
Aalb_oocyte_rep_c36433_trimmed	Conserved hypothetical protein (AAEL008645)	DI	1.33	0.000	unknown
Aalb_oocyte_GH79BIP01BENVH_trimmed	Bhlhzip transcription factor bigmax	DI	0.87	0.010	DNA replication/ transcription
Aalb_oocyte_rep_c3449_trimmed	GPCR Methuselah Family	a priori	0.88	0.004	stress response
Aalb_oocyte_rep_c40438_trimmed	Receptor for activated C kinase, putative (rack1)	DI	0.86	0.003	endocrine signaling
Aalb_oocyte_rep_c18194_trimmed	Conserved hypothetical protein (AAEL012019)	DI	0.92	0.010	unknown
Aalb_oocyte_rep_c431_trimmed	phosphatidylethanolamine-binding protein (pebp)	a priori	0.88	0.000	morphogenesis

Information on each gene verified as differentially expressed is shown. Putative homologs were derived from BLAST searches (see Methods). "454 category" refers to the differential expression status assigned to the EST based on read counts: DI or NDI, significantly over-expressed under DI or NDI conditions, respectively; and a priori, not DE based on 454 read counts, but of a priori interest based on previous diapause research. Fold-change calculations are based on qPCR results, and were calculated as log2(DI-NDI), where DI and NDI are the average mRNA abundance for DI and NDI treatments, respectively (see Methods). The corrected p-value is derived from Student''s t-test of mRNA abundances derived from qPCR reactions.

ISSN: 1471-2164