Distribution of DIRS1-like elements among the eukaryote groups tested. Species phylogeny was redrawn from [42–45]. Chromalveolate species are shown with red branches, excavates with purple branches, plants with green branches, and unikonts with blue branches. Groups in which DIRS1-like elements were detected and confirmed are shaded in yellow. Groups in which the presence of DIRS1-like element remains uncertain are shaded in green. In each group, we include in parentheses the number of species harboring DIRS1-like sequences (in blue) compared to the number of species analyzed (in red), as well as the number of species not screened in this study in which DIRS1-like retrotransposons were previously reported (in purple). B: Bilateria, D: Deuterostomia, F: Fungi.