Figure 4

EMSA with anti-NonO and anti-SFPQ antibodies. (A) EMSA experiment using the LTSM-positive RPL36 probes and different molar excesses of specific anti-NonO and anti-SFPQ antibodies alone or in conjunction (AB). The first lane contained the labelled probe; in the following lanes nuclear extracts (NE) were added. SC indicates unlabeled specific competitor probes and UC unspecific LTSM-negative RPL13A competitor probes. Specific binding is indicated by a black arrowhead. (B) Quantitative analysis of the specific binding in EMSA experiments. Statistical relevance refers to the specific RPL36 signal (* p ≤ 0.1/** p ≤ 0.05 in two-sided t-test, n = 6).