Table 3 Quantitative PCR Gene and Primer Information.
From: Generation of a reference transcriptome for evaluating rainbow trout responses to various stressors
Gene | Gene Symbol | Primer Sequences | Amplicon Size (bp) |
|---|---|---|---|
Caspase 3 | casp3 | TTTGGGAGTAGATTGCAGGG TGCACATCCACGATTTGATT | 52 |
Caspase 8 | casp8 | CAGCATAGAGAAGCAAGGGG TGACTGAGGGGAGCTGAGTT | 93 |
P53 | p53 | GTGGAATTTGATCCGAGTCTGT AGTGTCCAGGGTAGAAATGGAG | 78 |
Nuclear protein 1 | nupr1 | CGAAGAAGCACACTACGATCAA TCAGTCCGATTTCTCTCTTGGT | 98 |
Heat shock | Â | Â | 167 |
protein 70 b | hsp70b | AGGCCCAACCATTGAAGAGA GCAATGTCCAGCAATGCAATA | Â |
NaKATPalpha1a | nakatpa1a | GTGACGGTGAGAAGAAGAACATC GGCAGAGACGATACGCAAAT | 107 |
NaKATPalpha1b | nakatpa1b | GTCTTCCTGGGCGTGTCTTT CTCTGGCACATTAGCAACGAT | 105 |
NaKATPalpha1c | nakatpa1c | ATCGTGACTGGTGTTGAAGAAG GAAGAAGAGGAAGGGTGTGATTTC | 108 |
NaKATPalpha3 | nakatpa3 | GGCATCACCTTCTTCATCCT ACAGTAGCCAGTAAGCCCTCAG | 113 |
β-actin | Bact | GCCGGCCGCGACCTCACAGACTAC CGGCCGTGGTGGTGAAGCTGTAAC | 73 |
Elongation factor | Â | Â | 95 |
1α | 1f1a | CATTGACAAGAGAACCATTGA CCTTCAGCTTGTCCAGCAC |  |