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Figure 2 | BMC Genomics

Figure 2

From: H2B ubiquitylation is part of chromatin architecture that marks exon-intron structure in budding yeast

Figure 2

H2B ubiquitylation marks introns and exons. (A-C) Localization of H2BK123ub1 across the coding region of all intron-containing, RP and non-RP genes. The DNA sequences of exon1, intron and exon2 of all intron-containing genes in yeast were divided into 10, 30 and 40 bins, respectively. The composite of the averaged H2BK123ub1 level was aligned in the order: exon1-intron-exon2. (D-F) Nucleosome occupancy across the coding region of all intron-containing RP and non-RP genes. The composite profiles were analyzed as in (A-C). The average occupancy of nucleosomes in introns and exons was derived from data in Jiang and Pugh (2009) [42] and calculated as described in Materials and Methods. (G) H2B and (H) H3 occupancies at several intron- containing ribosomal protein (RP) genes were assayed by ChIP using an anti-Flag antibody to detect FLAG-H2B and an antibody against the C-terminus of H3. The relative H2B and H3 occupancies were calculated by comparison to the occupancy of these histones at the INT-V region.

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