Skip to main content
Figure 3 | BMC Genomics

Figure 3

From: H2B ubiquitylation is part of chromatin architecture that marks exon-intron structure in budding yeast

Figure 3

H2BK123ub1, H3K36me3, H3K4me3, and H3K79me3 levels in introns and exons of ribosomal protein genes. (A) ChDIP was performed to detect the level of HA-ubiquitin tagged Flag-H2B. Antibodies against (B) H3K36me3 (C) H3K4me3 and (D) H3K79me3 were used in ChIP to detect the levels of these three marks. Probe-based PCR primers were designed to amplify promoter (P), intron, exon, and 3' intergenic regions for the genes analyzed. H2BK123ub1 levels were normalized to H2B, and H3K36me3, H3K4me3 and H3K79me3 levels were normalized to H3. In (B) the levels of H3K36me3 were also measured in an htb-K123R mutant (K123R) that lacks H2BK123ub1. Error bars represent the standard deviation of at least three independent experiments.

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us