Figure 4

Comparison of N50 values assembled with various sequencing depth combination of 454 and Illumina reads. The assemblies were achieved by using the two step approach (Newbler for 454 data, Velvet for Illumina data for initial assembly followed by assembly with MIRA, for details, see materials and methods), with various sequencing depth combination of 454 sequence reads and Illumina sequence reads. Red circle indicates the sequence depths at which additional sequencing started to loss power for effective sequence assembly.