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Table 2 Comparison of assembly output with 454 reads and Illumina reads using different assemblers with different K-values.

From: A pilot study for channel catfish whole genome sequencing and de novo assembly

 

Assembler

K-value/Seed length

N50 (bp)

Maximum contig size (bp)

Average contig size (bp)

Number of contig

454

 

10

8,497

28,747

3,387

290

  

11

7,524

28,723

3,457

284

  

12

8,164

26,457

3,520

279

 

Newbler

13

8,670

31,603

3,478

283

  

14

8,635

28,741

3,523

279

  

15

8,164

35,657

3,487

282

  

16

8,674

28,736

3,486

282

  

25

653

4,678

437

1,787

  

35

736

3,717

468

1,645

  

45

744

3,958

505

1,409

 

Velvet

55

731

3,515

513

1,213

  

65

718

2,730

556

943

  

75

691

3,060

574

757

  

85

621

3,538

584

622

  

95

595

3,291

600

488

 

MIRA

 

1,331

8,705

856

1,254

 

CLC Genomics Workbench

 

1,239

7,621

988

1,220

Illumina

 

21

4,196

23,500

1,242

954

  

25

5,678

40,818

1,495

809

  

29

6,319

35,339

1,703

705

  

33

4,745

32,597

1,744

659

  

37

4,507

22,638

1,725

637

 

Velvet

41

4,355

15,944

1,712

602

  

45

3,619

19,463

1,677

579

  

49

3,605

18,297

1,849

511

  

53

3,381

18,243

1,813

518

  

57

3,025

15,219

1,631

552

  

61

3,388

15,148

2,011

390

  

30

2,296

13,010

1,027

1,013

  

35

2,493

16,400

1,132

933

  

40

3,647

20,868

1,439

716

 

ABySS

45

4,463

20,727

1,681

578

  

50

5,481

22,931

2,084

453

  

55

5,459

23,830

2,316

401

  

60

6,870

22,594

2,496

386

  

64

4,285

16,790

1,722

524

 

CLC Genomics Workbench

 

823

8,964

454

3,572