Figure 4

Effect of cold-adaptation (25°C/72 h) and cold storage (0°C/48 h) on thioredoxin (Trx), thioredoxin reductase (TrxR) and GST (glutathione S transferase) activity. (A) Cold-adaptation increases Trx activity before or after 0°C storage (*P < 0.05 vs. 37°C control; **P < 0.001 vs. 37°C + 0°C/48 h + RW; n = 5 experiments). (B) TrxR activity is greater in cold-adapted cells following 0°C storage and rewarming (*P < 0.05 vs. 37°C + 0°C/48 h + RW; n = 4 experiments). (C) GST activity in cold-adapted cells following 0°C storage and rewarming exceeds that of control cells (*P < 0.05 vs. 37°C + 0°C/48 h + RW; n = 4 experiments). Open bars represent cell cold-adapted at 25°C for 72 h; closed bars represent cells maintained at 37°C for 72 h.