Proteomic analysis of endothelial cold-adaptation

BMC Genomics

Table 1 Changes in protein expression following cold-adaptation as determined by LC/MS-based label-free protein quantitative analysis.

Protein priority	Peptide ID confidence	Multiple sequences	Number of proteins	Number significant changes	Maximum absolute fold change	Number increasing at 25°C	Number decreasing at 25°C
1	High	Yes	440	169 (15.5%)	1.70	73	96
2	High	No	364	50 (4.6%)	1.92	12	38
3	Moderate	Yes	7	4 (0.04%)	1.23	0	4
4	Moderate	No	278	81 (7.4%)	2.37	17	64
Overall			1089	304 (27.9%)	2.37	102 (9.4%)	202 (18.5%)

A total of 1089 proteins were identified by LC/MS and 27.9% of these had significantly increased or decreased in abundance following 25°C/72 h. Priorities 1-4 were assigned based on confidence in peptide identification (High and Moderate) and whether or not there are multiple amino acid sequences (Yes or No) identified. Please see references [24] and [26] for more details about how the protein priority is determined.

ISSN: 1471-2164