Figure 3

Relative enrichment of newly identified TREs determined by ChIP-PCR. The top half of each section shows the amplicons run on an agarose gel, and the bottom half shows relative enrichment of the immunoprecipitated (IP) samples and the total input (TI) samples when compared to β-actin enrichment. ChIP-PCR validation of negative (β-actin) and positive (Mlxipl) controls are presented in section (A). Sections (B) to (E) show enrichment of Tor1a, H01Rik (2310003H01Rik), Hectd3 and Slc25a45. Asterisks (*) denote a significant difference, p ≤ 0.05, determined by student t-test. The mean enrichments (± standard deviations) are also presented for each section. All presented immunoprecipitated enrichments were significant (p ≤ 0.05 determined by student t-test) when compared to total input, except for β-actin.