Figure 3

Nucleotide and amino acid frequency ratios between the 5'- and 3'-ends of genes. The 21 amino acids from the 5'-ends and 20 amino acids from the 3'-ends of all C. albicans genes (and the corresponding nucleotides) were compared by calculating the 5'/3' frequency ratio of each nucleotide (a) or amino acid (b). Bars in (b) are colored by the average GC-skew of the codons that encode for the corresponding amino acid (see colorbar), demonstrating that negatively GC-skewed amino acids are enriched at the 5'-ends of genes while positively GC-skewed amino acids are enriched at the 3'-ends of genes; Methionine is an exception because it is the first amino acid of all genes.