Interchromosomal comparison between Btau and UMD. Graphic output of Circos software illustrating all DCMs larger than 100 kbp. DCMs are depicted as lines connecting the Btau chromosomes (left side on orange background) to the UMD chromosomes (right side on light blue background). The red lines are for DCMs tested by FISH. The black arrowheads point to the assembly supported by the FISH results. In the case of the DCM_3-13, the UMD chr13 is connected to two Btau chromosomes (3 and 13) by two lines: the gray dashed line indicates that the sequence is present in the corresponding Btau chromosome; the red line points to its duplication on Btau chr3. The upper box shows chr3 and chr13 ideograms on which are reported the bioinformatics positions of BAC CH240-123I9 (internal to the DCM_3-13 sequence; red dots) according to Btau and UMD and the positions of the two chromosome-specific BACs, in green for chr3 and blue for chr13. The BAC-FISH experiment reported on the right clearly shows that the sequence is present only on chr3 (for detail see text).