Analysis of polysome distributions of selected mRNAs. WT strain YAJ3 was grown under non-permissive conditions as described above and WCEs were resolved by sedimentation through sucrose gradients as in Figure 1B. The mRNAs indicated above each panel were quantified by qRT-PCR in 1 μg of RNA from the 80S (Mono), LP, and HP fractions. The resulting values were normalized to the levels of 18S rRNA quantified in the same samples and then multiplied by a factor corresponding to the proportion of total A280 units in the gradient present in the cognate fractions (80S, LP, or HP). The results for each fraction are plotted as a proportion of the total amount present in all three fractions combined.