Characteristics of genes exhibiting altered translational efficiencies on depletion of eIF4G. (A) Cellular functions enriched among mRNAs translated relatively better (TE4G/TEWT ≥1.4) or relatively worse (TE4G/TEWT ≤ 0.71) on eIF4G depletion. The degree of enrichment (Log2 N-fold enrichment) as compared to the frequency among all tested genes for significantly enriched or underrepresented functional categories (P < 0.05, Fisher's exact test, Bonferroni correction) is shown. Functional enrichment was analyzed using the MIPS Funcat system (http://mips.helmholtz-muenchen.de/genre/proj/yeast/Search/Catalogs/catalog.jsp) (B) Frequency of essential genes within the complete set of tested genes (All) and among genes with TE4G/TEWT ≥1.4 or TE4G/TEWT ≤ 0.71. The frequency of essential genes in the latter two categories deviate significantly from the frequency for all tested genes (Fisher's exact test; P < 0.05).