Multiple sequence alignment of the DBD domains of the Hsf protein family in maize. The definition of the Hsf numbers corresponds to the order of alignment. The multiple alignment results clearly show the highly conserved DBD domains among maize Hsf genes. The secondary structure elements of DBD (α1-β1-β2-α2-α3-β3-β4) are shown above the alignment. Cylindrical tubes represent α-helices and block arrows represent β-sheets.