Figure 4

Examples of amendments to the Ae. aegypti annotation supported by HMMSplicer results. Black bars in the top tracks represent the current gene annotations. Blue histograms in the second track represent the non-normalized coverage of RNA-seq reads at each position. The range of the histogram values shown in each view is depicted on the labeled y-axis of each RNA-seq track. Black boxes in the lower track represent splice-site predictions based on the RNA-seq reads using HMMSplicer determined in this study. Each function has a unique identifier listed below and its HMMSplicer score is listed in red. If multiple reads support a single junction, "junc = x" lists the number of supporting reads. This information provides evidence to link two islands of transcription as a single transcription event, therefore, exons of a common mRNA. All predicted junctions shown here also are supported by EST alignments. Genes are (A) AAEL006259; (B) AAEL010818; and (C) AAEL001774 and AAEL001759.