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Table 4 Differentially expressed proteins in ΔsRNA-Xoo 1 identified by MS

From: Identification and functional characterization of small non-coding RNAs in Xanthomonas oryzae pathovar oryzae

Spot ID protein name functions NCBI acc.no. mascot score Sequence coverage theoretical
MW(Da)/pI
Ratio ± SD
Down-regulated protein spots in sRNA- Xoo 1 mutant
B1 putative ABC transporter ATP-binding protein transport PXO_04409 448 56% 61740/5.37 11.57 ± 4.83
B2 glyceraldehyde-3-phosphate dehydrogenase metabolism PXO_02308 529 69% 36134/6.35 4.75 ± 0.57**
B3 argininosuccinate synthase amino acid biosynthesis PXO_00352 769 67% 45217/5.66 3.25 ± 0.56
B4 tryptophan repressor binding protein amino acid metabolism PXO_03044 438 52% 20167/6.05 2.83 ± 0.18**
B5 3-isopropylmalate isomerase large subunit amino acid biosynthesis PXO_02613 314 42% 51984/5.68 1.86 ± 0.21**
B6 N-acetylornithine carbamoyltransferase amino acid biosynthesis PXO_00353 314 65% 37582/6.07 1.7 ± 0.19**
B7 superoxide dismutase oxidation reduction PXO_00389 343 68% 22703/5.47 1.42 ± 0.03**
B8 septum site-determining protein MinD replication PXO_04464 435 46% 28837/5.21 1.57 ± 0.19**
B9 N-ethylammeline chlorohydrolase metabolism PXO_00380 263 62% 48943/5.19 ND
B10 2-isopropylmalate synthase amino acid biosynthesis PXO_02609 694 71% 53421/5.54 1.54 ± 0.15**
B11 translation elongation factor G transcription and translation PXO_04525 642 58% 75924/5.09 4.82 ± 0.42**
B12 dihydrolipoamide dehydrogenase amino acid metabolism PXO_01196 582 67% 50445/5.98 2.29 ± 0.54
B13 N-acylglucosamine 2-epimerase metabolism PXO_01231 811 77% 46884/5.27 2.23 ± 0.34**
B14 ATP synthase subunit alpha transport PXO_03111 777 48% 55391/5.38 2.23 ± 0.5
B15 phosphoenolpyruvate synthase Pyruvate metabolism PXO_00922 622 48% 86689/5.16 6.36 ± 1.69
B16 Polyribonucleotide nucleotidyltransferase metabolism PXO_01307 729 55% 75502/5.47 5.39 ± 0.93
Up-regulated protein spots in ΔsRNA- Xoo 1 mutant
B17 pyrroline-5-carboxylate reductase amino acid metabolism PXO_01991 301 51% 29364/4.93 1.48 ± 0.07**
B18 peptide deformylase protein synthesis PXO_04055 379 55% 17129/5.29 1.45 ± 0.02**
B19 outer membrane protein transport PXO_03097 343 32% 23707/5.27 1.43 ± 0.04**
B20 Chaperone protein dnaJ DNA replication PXO_01186 702 64% 41079/6.21 1.52 ± 0.14**
B21 hydrolase, carbon-nitrogen family metabolism PXO_06060 526 68% 30229/5.89 1.57 ± 0.12**
B22 hypothetical protein hypothetical protein PXO_01766 635 75% 16502/6.3 1.41 ± 0.01
  1. ND: not detectable in the 2-DE maps of wild-type or ΔsRNA-Xoo 1 mutant. SD: Standard Deviation. Statistical significance was determined using Student's two-tailed t test for unpaired means P < 0.05, P < 0.01**.