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Table 1 Summary of genome features in safe strains.

From: The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli

  pRK1 pRK2 W K-12 B Crooks
Accession & Version CP002186 CP0021857 CP002185 U00096.2 CP000819.1 CP000946.1
Chromosome size (Kbp) 102.5 5.36 4901 4640 4630 4746
G+C content 49.95 46.03 50.84 50.78 50.77 50.87
genes (pseudogenes) 117 (1) 16 (0) 4764 (91) 4493 (177) 4383 (67) 4409 (82)
CDSs 114 15 4482 4149 4209 4200
structural RNAs 3 1 191 172 107 128
   rRNAs 0 0 22 22 22 22a
   tRNAs (pseudo) 0 (0) 0 (0) 87 89 (3) 85 (0) 87 (1)
   other ncRNAs (pseudo) 2 (0) 1 (0) 82 61 (2) ND 19
Large Mobile Elements 0 0 10 10 11 9
   Prophage regions 0 0 7 8 10 8
   Integrative Elements 0 0 3 2 1 1
IS elements (pseudo) 2 (0) 0 (0) 18 (6) 41 (13) 50 (12) 39 (15)
LPS core type - - R1 K-12 R1 (IS1::waaT) R1
O antigen - - O6 O16 (IS5::wbbL) O7 (IS1::wbbD) O146 (IS1::wbwW)
H antigen    H49 H48 - ND
K antigen - - - - K5 (IS1::kfiB) -
Colanic acid (M-antigen) - - + + + +
  1. a ssrS is annotated as an rRNA in Crooks but in K-12 and W it is annotated as an ncRNA. It is included in this table as an ncRNA
  2. The total number of genes, tRNA, other ncRNAs and IS elements in each strain includes pseudogenes/pseudo-tRNAs etc.; the number of pseudo-elements in each case is in noted in brackets. A '+' means the element is present; a '-' means the element is absent. ND = not determined in annotation. Safe laboratory strains: W (ATCC 9637) and its plasmids pRK1 and pRK2; K-12 (MG1655); B (REL606); and Crooks (ATCC 8739). W is in phylogroup B1; K-12, B and Crooks are in phylogroup A.