Figure 5
From: GC content around splice sites affects splicing through pre-mRNA secondary structures
Free energy and GC content comparison between tissue-specific and non-tissue-specific alternative donor and acceptor sites in humans with boxplots. A is for the energy comparison between tissue-specific and non-tissue-specific splice sites in humans. The average free energy at the donor sites was -46.25 kcals/mol for tissue-specific and -41.20 kcals/mol for non-tissue-specific donor sites (Wilcoxon test, P < 2.2 × 10-16). At the acceptor site, the average energy was -40.65 vs. -39.98 kcals/mol (Wilcoxon test, P = 0.04). B is for the GC content comparison. The average GC content for the tissue specific donor sites was 0.56, and decreased to 0.51 for non-tissue-specific ones (Wilcoxon test, P < 2.2 × 10-16). Similar results were obtained at the acceptor sites (0.53 vs 0.51, Wilcoxon test, P < 4.9 × 10-5).