Table 4 List of differentially regulated genes in key functional groups.
Gene locus in EA 2018 | Gene locus in ATCC 824 | 9 h (Log2Ratio 2018/824) | 13 h (Log2Ratio 2018/824) | 17 h (Log2Ratio 2018/824) | 21 h (Log2Ratio 2018/824) | 24 h (Log2Ratio 2018/824) | 30 h (Log2Ratio 2018/824) | Product Name |
|---|---|---|---|---|---|---|---|---|
Carbohydrate transportant and metabolism | Â | Â | Â | Â | Â | |||
CEA_G0343 | CA_C0332 | -2.97436 | -3.22501 | -2.37758 | -2.08064 | -2.57556 | -1.49158 | Beta-mannanase |
CEA_G0501 | CA_C0490 | 2.331505 | 2.410311 | 2.244172 | 2.361906 | 2.703307 | 2.474026 | sugar kinase, N-terminal region - uncharacterized protein |
CEA_G0552 | CA_C0539 | -3.20909 | -3.00324 | -2.93013 | -2.29917 | -3.34939 | -3.01922 | ChW repeat-containing mannanase ManB |
CEA_G0553 | CA_C0540 | -3.097 | -3.03398 | -2.7227 | -2.28306 | -3.3665 | -2.72934 | ChW repeat-containing mannanase ManB |
CEA_G1677 | CA_C1664 | 1.744408 | 2.013214 | 2.53088 | 1.134795 | 1.816259 | 2.062687 | glycogen phosphorylase |
CEA_G2012 | CA_C1997 | 3.052977 | 3.503142 | 3.004392 | 2.8712 | 2.075025 | 1.372718 | glycosyltransferase |
CEA_G2022 | CA_C2007 | 3.179932 | 3.308867 | 2.812136 | 2.509044 | 1.656072 | 1.958591 | glycosyltransferase |
CEA_G2528 | CA_C2514 | 2.588809 | 2.748177 | 3.625871 | 4.329215 | 2.708288 | 1.042021 | Beta galactosidase |
CEA_G2815 | CA_C2807 | 2.40568 | 2.124155 | 2.476058 | 2.653992 | 2.808752 | 1.737289 | endo-1,3(4)-beta-glucanase family protein 16 |
CEA_G2818 | CA_C2810 | 1.835243 | 2.287891 | 2.800041 | 3.929157 | 3.739976 | 4.256289 | glucoamylase family protein |
CEA_G3051 | CA_C3045 | -2.41601 | -2.05151 | -2.19775 | -2.37262 | -2.3321 | -2.16444 | PHP family hydrolase |
CEA_G3060 | CA_C3054 | -3.63818 | -4.38617 | -4.64833 | -5.02922 | -5.12371 | -3.50751 | phosphoheptose isomerase |
CEA_G3426 | CA_C3422 | -1.07396 | -2.79221 | -3.22016 | -2.15086 | -2.69786 | -1.37931 | sugar:proton symporter (xylulose) |
CEA_P0052 | CA_P0053 | 2.477697 | 2.868013 | 3.649329 | 3.695172 | 3.721454 | 5.987476 | xylanase |
CEA_P0053 | CA_P0054 | 4.25084 | 2.735168 | 2.447001 | 3.424303 | 3.598697 | 5.185082 | xylanase/chitin deacetylase family protein |
CEA_P0065 | CA_P0066 | 3.062167 | 1.145145 | 2.159875 | 4.902259 | 3.81378 | 2.953822 | mannose-specific phosphotransferase system component IIAB |
CEA_P0066 | CA_P0067 | 2.925021 | 1.579742 | 2.174524 | 4.883877 | 3.683632 | 2.826095 | mannose/fructose-specific phosphotransferase system component IIC |
CEA_P0067 | CA_P0068 | 3.006142 | 1.74298 | 2.611356 | 4.717371 | 3.502957 | 2.456002 | mannose-specific phosphotransferase system component IID |
CEA_P0115 | CA_P0116 | 1.777221 | 2.342324 | 2.902238 | 3.126694 | 3.321994 | 5.378643 | xylanase |
Amino acid transport and metabolism | Â | Â | Â | Â | Â | |||
CEA_G0180 | CA_C0176 | 2.56498 | 2.063235 | 2.36635 | 2.08086 | 3.251555 | 3.705152 | oligopeptide-binding protein, periplasmic component |
CEA_G0327 | CA_C0316 | -4.09229 | 6.429377 | 7.957548 | 6.881528 | 4.185199 | 2.037144 | ornithine carbomoyltransferase |
CEA_G0390 | CA_C0380 | -4.23921 | 5.06975 | 5.365591 | 5.142555 | 3.394778 | 2.099342 | periplasmic amino acid-binding protein |
CEA_G0984 | CA_C0973 | -4.67305 | 6.390879 | 8.78089 | 7.141168 | 3.889621 | 2.522783 | argininosuccinate synthase |
CEA_G0985 | CA_C0974 | -4.03279 | 6.033303 | 8.738086 | 7.66813 | 4.662246 | 3.462605 | argininosuccinate lyase |
CEA_G2392 | CA_C2377 | -4.81354 | -4.50847 | -4.89131 | -5.25303 | -5.6598 | -6.94396 | oligopeptide ABC-type transporter, periplasmic binding component (frameshift) |
CEA_G2403 | CA_C2388 | -3.653 | 6.58659 | 9.490741 | 7.214547 | 3.839977 | 1.277684 | acetylornithine aminotransferase |
CEA_G2405 | CA_C2390 | -4.029 | 7.044851 | 9.332151 | 8.593887 | 5.225523 | 1.729156 | N-acetyl-gamma-glutamyl-phosphate reductase |
CEA_G2406 | CA_C2391 | -3.63924 | 6.659273 | 9.430206 | 8.041522 | 4.889782 | 1.658329 | bifunctional ornithine acetyltransferase/N-acetylglutamate synthase protein |
CEA_G2531 | CA_C2517 | 2.601974 | 2.875402 | 3.244092 | 4.199853 | 5.407919 | 5.659197 | extracellular neutral metalloprotease, NPRE |
CEA_G3059 | CA_C3053 | -3.80847 | -4.46351 | -4.85998 | -5.20027 | -5.64176 | -4.16398 | histidinol phosphatase related enzyme |
CEA_G3625 | CA_C3618 | -2.33371 | 4.975742 | 4.579403 | 4.356899 | 3.79613 | 1.400104 | ABC-type polar amino acid transport system, ATPase component |
CEA_G3626 | CA_C3619 | -2.49499 | 4.94909 | 4.797836 | 4.831871 | 4.227636 | 1.349231 | amino acid ABC transporter permease |
CEA_G3627 | CA_C3620 | -2.79225 | 4.869307 | 2.77014 | 4.039679 | 3.923914 | 1.658129 | amino acid ABC transporter periplasmic-binding protein |
CEA_G3629 | CA_C3622 | 3.744723 | 1.616914 | -1.60643 | -2.30924 | -3.70552 | -4.20925 | benzoyl-CoA reductase/2-hydroxyglutaryl-CoA dehydratase |
CEA_G3648 | CA_C3641 | 3.877563 | 5.273677 | 6.532841 | 7.05381 | 6.150331 | 2.645978 | oligopeptide ABC transporter, ATPase component |
CEA_G3649 | CA_C3642 | 3.862767 | 5.064748 | 6.213521 | 7.100308 | 6.123778 | 2.328286 | oligopeptide ABC transporter, ATPase component |
CEA_G3651 | CA_C3644 | 3.540737 | 4.731217 | 4.796332 | 6.751584 | 6.151055 | 3.301733 | oligopeptide ABC transporter, permease component |
Lipid transport and metabolism | Â | Â | Â | Â | Â | Â | ||
CEA_P0077 | CA_P0078 | -1.03663 | -2.39938 | -2.90422 | -5.61457 | -6.37659 | -6.669 | acetyl-CoA acetyltransferase |
CEA_G0500 | CA_C0489 | 2.843362 | 2.727643 | 3.104514 | 2.258672 | 2.789674 | 2.994569 | 4'-phosphopantetheinyl transferase |
CEA_G2024 | CA_C2009 | 3.02793 | 3.412819 | 2.753976 | 2.880761 | 2.465438 | 1.317947 | 3-hydroxyacyl-CoA dehydrogenase |
CEA_G2027 | CA_C2012 | 3.139119 | 3.519089 | 2.760385 | 2.466674 | 1.747722 | 1.295802 | enoyl-CoA hydratase |
CEA_G2023 | CA_C2008 | 3.21547 | 3.393795 | 2.65468 | 2.624962 | 1.849978 | 1.696553 | 3-oxoacyl-(acyl-carrier-protein) synthase |
CEA_G0825 | CA_C0814 | 3.447389 | 2.690101 | 3.341654 | 3.492821 | 2.738719 | 2.617536 | 3-oxoacyl- |
CEA_G3630 | CA_C3623 | 3.78405 | 1.698615 | -1.62094 | -2.13197 | -3.66189 | -4.47732 | 2-hydroxyglutaryl-CoA dehydratase activator |
Coenzyme transport and metabolism | Â | Â | Â | Â | Â | Â | ||
CEA_G2539 | CA_C2526 | -5.80423 | -4.29934 | -2.18936 | -3.20732 | -2.04208 | -2.09088 | 6-pyruvoyl-tetrahydropterin synthase related protein |
CEA_G0110 | CA_C0109 | -1.93293 | -1.10289 | -4.51818 | -4.22606 | -3.40598 | -5.95312 | sulfate adenylyltransferase subunit 2 |
CEA_G2240 | CA_C2226 | 1.175604 | 1.630568 | 1.933437 | 3.44313 | 4.132037 | 3.011011 | branched-chain amino acid aminotransferase |
CEA_G2817 | CA_C2809 | 1.909506 | 2.362089 | 1.59545 | 3.297164 | 2.183049 | 1.029092 | HD superfamily hydrolase |
CEA_G2037 | CA_C2022 | 2.45013 | 1.945668 | 2.521958 | 2.427533 | 2.187431 | 3.238565 | molybdopterin biosynthesis protein MoaB |
CEA_G3633 | CA_C3626 | 2.627562 | 1.339719 | -1.38437 | -2.32524 | -3.57634 | -5.06491 | GTP cyclohydrolase I |
CEA_G2036 | CA_C2021 | 2.849463 | 2.144804 | 2.666979 | 4.297463 | 3.319713 | 3.863173 | molybdopterin biosynthesis protein MoeA |
CEA_G2009 | CA_C1994 | 3.136184 | 3.75827 | 3.408469 | 2.84809 | 1.881193 | 1.268359 | molybdopterin biosynthesis protein MoaB |
CEA_G2035 | CA_C2020 | 3.146626 | 3.515592 | 5.182665 | 4.677354 | 4.692789 | 4.012931 | molybdopterin biosynthesis protein MoeA |
CEA_G3631 | CA_C3624 | 3.416604 | 1.490697 | -1.38827 | -2.23319 | -3.83641 | -6.15768 | 6-pyruvoyl-tetrahydropterin synthase |
Signal transduction | Â | Â | Â | Â | Â | Â | Â | |
CEA_G0078 | CA_C0078 | -4.99228 | -8.71284 | -9.43594 | -9.21854 | -9.14927 | -7.97564 | putative accessory gene regulator protein |
CEA_G3328 | CA_C3325 | -1.00804 | -2.85267 | -4.3502 | -5.19446 | -4.39209 | -5.05262 | periplasmic amino acid binding protein |
CEA_G0921 | CA_C0909 | -2.84748 | -1.88833 | -1.93256 | -1.50557 | -2.64087 | -2.89144 | methyl-accepting chemotaxis protein |
CEA_G2085 | CA_C2071 | 2.455498 | 2.263411 | 2.413649 | 1.803983 | 2.20351 | 2.550339 | Spo0A protein |
CEA_G2422 | CA_C2407 | 3.784844 | 1.475039 | 1.927948 | 1.823945 | 2.526403 | 2.502499 | CheY-like domain-containing protein |
CEA_G0448 | CA_C0437 | 2.192653 | 1.378372 | 2.665966 | 1.827148 | 2.252286 | 2.342264 | sensory transduction histidine kinase |
CEA_G3025 | CA_C3019 | -2.65448 | 3.491201 | 3.449102 | 2.735229 | 1.336433 | 1.266833 | sensory transduction protein |
CEA_G0296 | CA_C0289 | 1.792323 | 1.978035 | 2.438903 | 2.751395 | 3.435751 | 3.052853 | response regulator |
CEA_G2782 | CA_C2774 | 1.625932 | 1.895382 | 3.060796 | 2.846554 | 2.092881 | 1.726376 | methyl-accepting chemotaxis protein |
CEA_G0334 | CA_C0323 | 3.362504 | 2.662176 | 2.652177 | 3.222052 | 4.952742 | 2.037345 | sensory transduction histidine kinase |
CEA_G3627 | CA_C3620 | -2.79225 | 4.869307 | 2.77014 | 4.039679 | 3.923914 | 1.658129 | amino acid ABC transporter periplasmic-binding protein |
CEA_G0390 | CA_C0380 | -4.23921 | 5.06975 | 5.365591 | 5.142555 | 3.394778 | 2.099342 | periplasmic amino acid-binding protein |
Energy production and convertion | Â | Â | Â | Â | Â | Â | ||
CEA_G1083 | CA_C1072 | 3.271648 | 3.230747 | 4.254617 | 2.59071 | 2.723209 | 3.766376 | Fe-S oxidoreductase |
CEA_G2012 | CA_C1997 | 3.052977 | 3.503142 | 3.004392 | 2.8712 | 2.075025 | 1.372718 | glycosyltransferase |
CEA_G2015 | CA_C2000 | 2.963078 | 3.638083 | 2.745804 | 2.983214 | 2.587284 | 2.204476 | indolepyruvate oxidoreductase subunit beta |
CEA_G2016 | CA_C2001 | 2.832561 | 3.688549 | 3.007107 | 3.355002 | 2.718563 | 2.206461 | indolepyruvate ferredoxin oxidoreductase, subunit |
CEA_G2022 | CA_C2007 | 3.179932 | 3.308867 | 2.812136 | 2.509044 | 1.656072 | 1.958591 | glycosyltransferase |
CEA_G2025 | CA_C2010 | 3.282342 | 3.533526 | 2.84142 | 3.003615 | 2.50221 | 2.005231 | Fe-S oxidoreductase |
CEA_G2555 | CA_C2542 | 8.22595 | 5.876659 | 5.423124 | 5.135223 | 4.224852 | 4.280766 | FAD/FMN-containing dehydrogenase |
CEA_G2556 | CA_C2543 | 8.451735 | 6.100494 | 5.401602 | 5.24343 | 4.426238 | 3.987954 | electron-transferring flavoprotein large subunit |
CEA_G2557 | CA_C2544 | 7.904188 | 6.040402 | 4.848558 | 4.578106 | 4.214698 | 3.969443 | electron-transferring flavoprotein small subunit |
CEA_G3411 | CA_C3408 | -2.71993 | -3.64684 | -4.53359 | -4.7967 | -4.89809 | -4.45863 | NADH oxidase |
Cell mobility | Â | Â | Â | Â | Â | Â | Â | Â |
CEA_G0921 | CA_C0909 | -2.84748 | -1.88833 | -1.93256 | -1.50557 | -2.64087 | -2.89144 | methyl-accepting chemotaxis protein |
CEA_G3572 | CA_C3565 | 1.783589 | 1.966743 | 1.626123 | 1.303128 | 1.522492 | 1.56009 | cell adhesion domain-containing protein |
CEA_G3091 | CA_C3085 | 1.669031 | 1.879947 | 1.641572 | 3.329325 | 2.811493 | 2.558158 | TPR repeat-containing cell adhesion protein |
CEA_G3092 | CA_C3086 | 1.689695 | 1.7646 | 1.823344 | 3.167841 | 2.629106 | 2.423856 | cell adhesion domain-containing protein |
CEA_G2782 | CA_C2774 | 1.625932 | 1.895382 | 3.060796 | 2.846554 | 2.092881 | 1.726376 | methyl-accepting chemotaxis protein |
CEA_P0159 | CA_P0160 | 2.878477 | 3.739495 | 3.515178 | 3.50976 | 3.19802 | 3.547315 | cell-adhesion domain-containing protein |