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Table 3 Transcriptome coverage estimates: ESTcalc

From: De novo characterization of the gametophyte transcriptome in bracken fern, Pteridium aquilinum

Input Parameters ESTcalc estimate Actual
Number of technologies 1 1
Technology 454 GS-FLX 454 GS-FLX (Titanium)
Library type normalized normalized
MB/Plate 254 254.0076
Reads/Plate 681,722 681,722
BP/Read (mean) 372.6 372.6
Predicted assembly statistics   
Total Assembled Sequence (MB) 26.2 30.97
Unigene count 32,044 56,256
Mean unigene length (bp) 819 547.23
Mean unigene length (longest unigene per gene, bp) 1,143 --
Singleton yield (%) 19 17.2400
Percent transcriptome (%) 87 --
Percent of genes tagged (%) 100 --
Percent of genes with 90% coverage (%) 69.8 --
Percent of genes with 90% coverage by largest unigene (%) 56.4 --
Percent of genes with 100% coverage (%) 23.7 --
Percent of genes with 100% coverage by largest unigene (%) 22.2 --
  1. Estimates of transcriptome coverage based on simulations modeled using the Arabidopsis thalliana floral transcriptome [57].