Table 3 Characteristics of signal peptidases and target signal peptides
From: Genome-wide protein localization prediction strategies for gram negative bacteria
Model Signal Peptidase | Example Protein | Merops Family & domains | Translocation system | Signal peptide domain | Example substrates | Domains | Comments |
|---|---|---|---|---|---|---|---|
N-terminal Processing | |||||||
PilD PulO GspO | SO_0414 | A24A | T2aSS | pfam07963 | GspGHIJK | pfam02501 pfam03934 pfam08334 pfam12019 | Signal peptides similar, except ones in IVb pili are longer (~25 aa) than others (~7 aa), GspK, PilX, PilW are not detected by pfam07963 |
| Â | Â | Â | T2bSS | Â | type IVa pili | Â | Â |
| Â | Â | Â | Â | Â | type IVb pili | Â | Â |
TadV | Spea_2010 | A24A | T2cSS | Â | Flp, TadEF | Â | Â |
LspA | SO_3531 | A08 pfam01252 | Sec | Â | Lipo-proteins | Â | Â |
| Â | Â | Â | Tat | TIGR01409 pfam10518 | Â | Â | Â |
NA1 | SO_A0049 | C39 | T1SS | TIGR01847 | class Ia & IIa-b bacteriocin, microcins | pfam01721 pfam10439 pfam10439 | The signal peptidase activity is encoded in the permease component of the T1SS system that exports the bacteriocin |
LepB | SO_1347 | S26A pfam00717 | Sec | Â | exported proteins | Â | Â |
| Â | Â | Â | Tat | TIGR01409 pfam10518 | Â | Â | Â |
C-terminal processing | |||||||
? | Â | Â | T4bSS - IncF | Â | TraA | Â | mature TraA is about ~68 aa in length with two TM spans that circularizes |
TrhF | Shewana3_4209 | S26A | T4bSS - IncH | Â | TrhA HdtZ | Â | Substrates have Sec signal peptide that is cleaved by LepB |
TraF | Sputw3181_1142 | S26A | T4bSS - IncJ | Â | TraA | TIGR02758 | Â |
TraF | Shewana3_1267 | S26C pfam00717 | T4bSS - IncP | Â | TrbC | pfam04956 | Â |