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Table 1 Sequencing, quality control and assembly of the two libraries

From: Effective gene collection from the metatranscriptome of marine microorganisms

 

Non-normalized

Normalized

 

Number of reads

607,490

572,233

Raw data

Average length

309.2bp

275.8bp

 

Total base pairs

187.9Mbp

157.8Mbp

 

Number of reads

483,335

373,627

Quality control

Average length

333.5bp

323.2bp

 

Total base pairs

161.0Mbp

120.8Mbp

 

Full-length

45,064

49,121

Assembly

Contig

53,324

32,440

 

Singlet

118,251

97,124

Final

Total number of genes

216,639

178,685

 

Total base pairs

73.7Mbp

57.3Mbp

  1. Raw data was produced on a Roche GS FLX sequencer. The quality control process removed low-quality sequences and vectors. After the identification of full-length genes, the assembly process classified contigs and singlets. The total number of genes represents the sum of full-length genes, contigs, and singlets.